CLINICAL SERVICES

Histology & Immunohistochemistry Laboratory

Laboratory Services

 

 

Immunohistochemistry
Immunohistochemistry is a technique for localizing and visualizing an antigen in a tissue section by using an antibody specific for the target antigen. The immunohistochemistry procedure consists of tissue preparation, antibody incubation, and a series of detection reactions. Technically, when the procedure is performed on cells (cell smears, cytocentrifuge preps, etc.) rather than tissue sections, it is termed immunocytochemistry, but in practice the two terms are often used interchangeably.


ANTIBODY COMMON APPLICATIONS CPT Code
Alpha-1Antichymotrypsin, polyclonal, IHC Histocytic Marker 88342
Alpha-Synueclein, polyclonal, IHC Alzheimer Disease 88342
ACTH (Adreno Corticotrophic Hormone), polyclonal, IHC Pituitary Tumors 88342
Actin Muscle, smooth, monoclonal, IHC Smooth Muscle 88342
ADH-5, (CK5/14+p63+CK7/18), monoclonal, IHC Subtype of breast lesions 88342
AFP (Alpha-Fetoprotein), polyclonal, IHC Hepatoma, Germ Cell Tumor 88342
ALK-1, monoclonal, IHC ALK-1 Positive Lymphoma 88342
Annexin, monoclonal, IHC Hairy cell leukemia 88342
Ber EP4, monoclonal, IHC Mesothelioma vs Adenocarcinoma 88342
B 72.3, monoclonal, IHC Mesothelioma vs Adenocarcinoma 88342
Bcl-2, monoclonal, IHC Lymphoma Typing, Soft Tissue Tumors 88342
Bcl-6, monoclonal, IHC Lymphoma Typing 88342
BOB-1, polyclonal, IHC B-chronic lymphocytic leukemia, mantle cell lymphoma, follicular lymphoma, marginal zone lymphoma, plasmacytoma, Burkitt lymphoma, diffuse large cell lymphoma, diffuse large B-cell lymphoma, T-cell rich B-cell lymphoma, and nodular lymphocyte predominant Hodgkin lymphoma. 88342
CA19-9, monoclonal, IHC Metastatic Carcinoma Unknown Primary 88342
CA125, monoclonal, IHC Mesothelioma vs. Adenocarcinoma 88342
CAM5.2, monoclonal, IHC Adenocarcinoma 88342
Calretinin, monoclonal, IHC Medullary carcinoma thyroid 88342
Calcitonin, polyclonal, IHC Medullary carcinoma thyroid 88342
Calponin, monoclonal, IHC Smooth Muscle differentiation, myoepithelial cells 88342
C-Kit, CD117, monoclonal, IHC Gastrointestinal stromal tumors mast cells 88342
c-erbB-2, Her 2, monoclonal, IHC Breast Cancer 88342
CEA (Carcinoembryonic Antigen),monoclonal, IHC Adenocarcinoma, metastatic carcinoma unknown primary 88342
CD1a, monoclonal, IHC Thymic T-cells, thymoma, langerhans cells 88342
CD2, monoclonal, IHC Lymphoma/leukemia typing 88342
CD3, monoclonal, IHC Lymphoma/leukemia typing 88342
CD4, monoclonal, IHC Lymphoma/leukemia typing 88342
CD5, monoclonal, IHC Thymic carcinoma 88342
CD7, monoclonal, IHC Lymphoma/leukemia typing 88342
CD8, monoclonal, IHC Lymphoma/leukemia typing 88342
CD10, monoclonal, IHC Lymphoma typing, metastatic carcinoma unknown primary 88342
CD15, monoclonal, IHC Lymphoma typing, mesothelioma vs. adenocarcinoma 88342
CD20, monoclonal, IHC Lymphoma/leukemia typing 88342
CD21, monoclonal, IHC Follicular dendritic cells, lymphoma typing 88342
CD23, monoclonal, IHC Lymphoma/leukemia typing 88342
CD25, monoclonal, IHC Systemic Mastocytosis 88342
CD30, monoclonal, IHC Lymphoma typing 88342
CD31, monoclonal, IHC Vascular differentiation 88342
CD33,monoclonal, IHC Identification of cells of myeloid and monocytic lineage, and leukemias and myeloproliferative disorders derived from these cells. 88342
CD34, monoclonal, IHC Soft tissue tumor classification, leukemia typing 88342
CD43, monoclonal, IHC Lymphoma/leukemia typing 88342
D45RB, monoclonal, IHC Recognition of lymphohematopoetic tumors 88342
CD45RO, monoclonal, IHC Lymphoma typing (same as UCHL-1) 88342
CD56, monoclonal, IHC Lymphoma typing, neuroendocrine differentiation 88342
CD57, monoclonal, IHC Lymphoma typing, neuroendocrine differentiation 88342
CD61, monoclonal, IHC Micromegakaryocytes in myelodysplastic syndrome, acute myeloid leukemia with multilineage dysplasia, acute megakaryoblastic leukemia, and myeloproliferative neoplasms 88342
CD68, monoclonal, IHC Histocytic/ myeloid/monocytic marker 88342
CD71,monoclonal, IH Erythroid leukemia, benign erythroid proliferative disorders, and myelodysplastic syndrome 88342
CD79a, monoclonal, IHC Lymphoma/leukemia typing 88342
CD99, monoclonal, IHC Soft tissue tumor classification 88342
CD123, monoclonal, IHC Lymphoma/leukemia typing 88342
CD138, monoclonal, IHC Plasma Cells (also stains many epithelial tumors) 88342
CD 207, Langerin, monoclonal, IHC Detect Langerin in cells and tissues 88342
CDK4, monoclonal, IHC Liposarcoma 88342
Chromogranin, monoclonal, IHC Neuroendocrine differentiation 88342
CLA (Cutaneous Lymphocyte Antigen, Rat, IHC Cutaneous Lymphocytes 88342
Clusterin, monoclonal, IHC Anaplastic Large Cell Lymphoma 88342
CMV (Cytomegalovirus), monoclonal, IHC Cytomegalovirus infection 88342
C-MYC, rabbit monoclonal, IHC Burkitt lymphoma 88342
CXCL-13, monoclonal, IHC Angioimmunoblastic T-cell lymphoma 88342
Cyclin D1, monoclonal, IHC Mantle cell lymphoma 88342
Cytokeratin 5/6, monoclonal, IHC Distinguish lung squamous cell carcinoma from lung adenocarcinoma and large cell carcinoma 88342
Cytokeratin 7, monoclonal, IHC Metastatic carcinoma unknown primary 88342
Cytokeratin 20, monoclonal, IHC Metastatic carcinoma unknown primary 88342
D2-40, monoclonal, IHC Lymphatic invasion of a variety of cancers 88342
Desmin, monoclonal, IHC Smooth and skeletal muscle differentiation 88342
EBER, ISH Epstein Bar Virus 88368
E-Cadherin, monoclonal, IHC Lobular vs. Ductal breast carcinoma 88342
EGFR, monoclonal, IHC Sweat gland carcinoma, squamous tumors 88342
EMA (Epithelial Membrane Antigen), monoclonal, IHC Metastatic carcinoma of unknown primary, lymphoma typing 88342
Epstein Barr Virus (LMP) , monoclonal, IHC Epstein Bar Virus 88342
Estrogen Receptor, rabbit monoclonal, IHC Breast cancer prognostic marker, metastatic carcinoma unknown primary 88342
Factor VIII-Related Antigen, polyclonal, IHC Megakaryocytic marker, endothelial marker 88342
Factor XIII-A-subunit, polyclonal, IHC Dermatofibroma vs. DFSP 88342
Fascin, monoclonal, IHC Hodgkin's Lymphoma 88342
FSH (Follicle Stimulating Hormone), polyclonal, IHC Pituitary tumors 88342
Gastrin, polyclonal, IHC Gastrinomas 88342
GCDFP-15(Gross Cystic Disease Fluid Protein-15), monoclonal, IHC Breast Cancer 88342
GFAP (Glial Fibrillary Acid Protein), monoclonal, IHC Glial tumors, salivary gland tumors 88342
GH (Growth Hormone), polyclonal, IHC Pituitary tumors 88342
Glucagon, polyclonal, IHC Pancreatic endocrine tumors 88342
Glycophorin A, monoclonal, IHC Characterize erythroid cell development and in the diagnosis of erythroid leukemia 88342
Glypican-3 (GPC-3), monoclonal, IHC Hepatocellular carcinoma (HCC) 88342
HBME-1, monoclonal, IHC Classification of mesotheliomas 88342
HCG-Beta (Human Chorionic Gonadotropin), polyclonal, IHC Choriocarcinoma 88342
Helicobacter Pylori, polyclonal, IHC Helicobacter Pylori 88342
Hemoglobin A, polyclonal, IHC Erythroid differentiation in various myeloproliferative and dysmyelopoietic syndromes 88342
Hepar, monoclonal, IHC Hepacellular carcinoma 88342
Herpes Simplex Virus Type I, polyclonal, IHC Herpesvirus I and II infection 88342
Herpes Simplex Virus Type II, polyclonal, IHC Herpesvirus I and II infection 88342
HHV-8, IHC Kaposi sarcoma 88342
HMB45 (Melanoma) , monoclonal, IHC Melanoma marker, angiomyolipoma 88342
IgA (Alpha Chains & Secretary Component),polyclonal, IHC Plasma cell subclassification 88342
IgG (Gamma Chains), polyclonal, IHC Plasma cell subclassification 88342
IgG4, monoclonal, IHC IgG4 systemic sclerosing disease 88342
IgM (Mu Chains), polyclonal, IHC Plasma cell subclassification 88342
IgD, polyclonal, IHC Plasma cell subclassification 88342
Inhibin-A, monoclonal, IHC Differential diagnosis of intra- uterine vs. ectopic pregnancy in endometrial curettings 88342
Insulin, monoclonal (GP) , IHC Pancreatic endocrine tumors 88342
Keratin 903, monoclonal, IHC Prostate marker 88342
Kappa Light Chains, polyclonal, IHC Plasma cell clonality 88342
Ki67, monoclonal, IHC Proliferative fraction, fat cells 88342
Lambda Light Chains, polyclonal, IHC Plasma cell clonality 88342
Laminin, monoclonal IHC Skeletal muscle 88342
LH (Lutenizing Hormone), polyclonal, IHC Pituitary tumors 88342
Lysozyme (Muramidase), polyclonal, IHC Myeloid and histiocytic marker 88342
Mammoglobin, monoclonal, IHC Breast cancer 88342
MART-1, monoclonal, IHC Melanoma marker, angiomyolipoma 88342
Mast Cell Tryptase, monoclonal IHC Mast Cell Tumors 88342
MDM2, monoclonal, IHC Liposarcoma or Breast cancer 88342
MLH-1, monoclonal, IHC Colon adenocarcinoma 88342
MSH-2, monoclonal, IHC Colon adenocarcinoma 88342
MSH-6, monoclonal, IHC Colon adenocarcinoma 88342
Myogenin, monoclonal, IHC Rhabdomyosarcoma 88342
Myosin, monoclonal, IHC Skeletal muscle 88342
MUM-1, monoclonal, IHC Lymphoma marker 88342
Myeloperoxidase, polyclonal, IHC Acute leukemia 88342
Napsin A, polyclonal, IHC Lung adenocarcinoma 88342
Neurofilament, monoclonal, IHC Neural tumors 88342
NSE (Neuron Specific Enolase), monoclonal, IHC Pancreatic endocrine tumors 88342
OCT-2, polyclonal, IHC Lymphoma marker 88342
OCT 3/4, monoclonal, IHC Germ cell marker 88342
P16, monoclonal, IHC Cervical dysplasia (correlates with high risk HPV) 88342
P21, monoclonal, IHC Gastric carcinoma, non-small cell lung carcinoma, thyroid carcinoma 88342
p53,(DO7), monoclonal, IHC Breast Carcinoma 88342
P63, monoclonal, IHC Prostatic basal cells- Prostate marker 88342
Pan-Actin Muscle (HHF35), monoclonal, IHC Smooth and Skeletal Muscle 88342
Pan Keratin( AE1/AE3+ PCK26),monoclonal, IHC Epithelial tumors 88342
Pan-Melanoma Cocktail (HMB-45, Mart-1, Tyrosianase), monoclonal, IHC Melanoma 88342
Parathyroid Hormone (PTH), polyclonal, IHC Parathyroid tumors 88342
Pax 5, monoclonal, IHC B-Cell Lymphoma 88342
PD1, monoclonal, IHC Follicular Helper T-Cells 88342
PIN-4 Cocktail, (CK5+CK14+p63+P504S), IHC Prostate marker 88342
Placental Alkaline Phosphatase, polyclonal, IHC Germ Cell Tumor 88342
PMS2, monoclonal, IHC Colon Adenocarcinoma 88342
Polyoma Virus, monoclonal, IHC Polyoma Virus – SV-40 88342
Progesterone Receptor, rabbit monoclonal, IHC Breast prognostic marker 88342
Prolactin, polyclonal, IHC Pituitary tumors 88342
PSA (Prostate Specific Antigen), monoclonal, IHC Prostate marker 88342
PSMA, polyclonal, IHC Prostate marker 88342
S100, monoclonal, IHC Spitz nevus 88342
Seratonin, monoclonal, IHC Carcinoids 88342
Somatostatin, polyclonal, IHC Pancreatic endocrine tumors 88342
Sox-11, polyclonal, IHC Blastoid Mantle Cell Lymphoma 88342
Spirochete ,polyclonal, IHC Spirochete 88342
Synaptophysin, monoclonal, IHC Neuroendocrine differentiation 88342
TCL-1A, monoclonal, IHC Leukemia 88342
TCRbf1,monoclonal, IHC Lymphoma typing 88342
TCR Gamma 3.2, monoclonal, IHC Characterize T-cells in lymphomas and inflammation 88342
TIA-1, monoclonal, IHC Lymphoma typing 88342
TDT, polyclonal, IHC Lymphoma/leukemia typing 88342
TTF-1, monoclonal, IHC Lung & thyroid marker, also some neuroendocrine 88342
Thyroglobulin, monoclonal, IHC Thyroid carcinoma 88342
TSH (Thyroid Stimulating Hormone), polyclonal, IHC Pituitary tumors 88342
Vimentin, monoclonal, IHC Metastatic Carcinoma Unknown Primary 88342

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