REQUISITION – Fungus Testing Laboratory requisitions are required. If your laboratory does not have one, it can be downloaded or printed from our website. Alternatively, please call and one will be faxed to you. Please indicate all pertinent information such as patient’s name, requesting entity, address, contact, phone/fax numbers, etc. Also indicate on the requisition the dosage, patient’s date/time of last dose and date/time of sample drawn.
UPLC/HPLC: - Set up Monday – Friday depending on the test and workload. Reports 24 hours following date of setup except for samples set up on Friday.
Sample TypePlasma or other biological fluids including CSF, bile, peritoneal fluid, dialysate or other biological fluids or tissues may be assayed for antifungal agents. Draw plasma samples using EDTA (lavender tubes). Do not use gel separator tubes as these may result in assay interference.
Plasma or other biological fluids including CSF, bile, peritoneal fluid, dialysate or other biological fluids or tissues may be assayed for antifungal agents. Draw plasma samples using EDTA (lavender tubes).
SPECIAL HANDLING– Samples should be frozen until shipment and sent to the Fungus Testing Laboratory overnight frozen (preferably on dry ice). Amphotericin B and micafungin samples should be protected from light.
SAMPLE AMOUNT – 0.5 to 1.0 ml of serum/plasma or other fluid.
SHIPMENT - Please ship samples to arrive Tuesday through Friday, as the University is closed on Saturday and Sunday. Pack samples in proper container and ship for overnight delivery on dry ice. Shipping fees are the responsibility of the sender. Note: Voriconazole samples may be shipped on cold packs.
RESULTS – Results are typically available 48 - 72 hours from receipt of sample, depending on when the sample is received. Samples received on Friday may not be completed until Tuesday of the following week. All reports are mailed after completion. We will also phone or FAX the report if so instructed on the requisition.
The antifungal drug levels division of the Fungus Testing Laboratory has the capability to quantitatively assay the following antifungal agents by either high performance liquid chromatography (HPLC) or ultra-performance liquid chromatography / mass spectrometry (UPLC/MS):
VORICONAZOLE– (VORI, HPLC) CPT 80299
POSACONAZOLE (POS, UPLC/MS) CPT 80299
ITRACONAZOLE (ITRA, UPLC/MS) CPT 80299
FLUCONAZOLE (UPLC/MS) CPT 80299
AMPHOTERICIN – B (AMB, HPLC) CPT 80299
MICAFUNGIN (MICA, HPLC) CPT 80299
CASPOFUNGIN – (CASPO, HPLC) CPT 80299
ANIDULAFUNGIN – (ANID, HPLC) CPT 80299 (Intermittent Testing)