The Flow Cytometry Laboratory provides complete immunophenotypic analysis by multi parameter flow cytometry and detection of terminal deoxynucleotidase (TdT) by immunofluorescence staining. The assays are of value in the diagnosis and surveillance of Leukemia (acute and chronic), Lymphoma, and immunodeficiency and immune altered states.
Flow cytomoetry and TdT staining are useful in the diagnosis and classification of acute leukemia, the diagnosis and classification of non-Hodgkin's lymphoma and the detection of residual leukemia or lymphoma following therapy. Analysis by the Flow Cytometry Laboratory includes morphologic evaluation by a pathologist with experience in hematopathology, selection of a suitable panel of immunophenotypic markers, notification of results by telephone within 24 hours of receipt of the specimen and mailed or faxed report including an interpretation of the data.
Flow cytometry is used for the monitoring of Human Immunodeficiency Virus (HIV) infection, diagnosis of congenital immunodeficiency states, and analysis of iatrogenic immunodeficiency. The Flow Cytometry Laboratory performs a complete immunophenotypic analysis following guidelines provided by the CDC. The report includes the perecentages and absolute counts for T-cell subsets, B-cell and natural killer (NK) cells, as well as a summary of any previous results.
Flow cytometry is of value in detecting and monitoring immune altered states associated with autoimmune diseases, exposure to toxic chemicals, and drug therapy.
Sample |
Requirements |
Storage / Shipping |
| Peripheral blood | 5-10 ml in EDTA | room temperature, less than 24 hours old |
| Bone marrow | 1-2 ml in EDTA | room temperature, less than 24 hours old |
| Pleural fluid | at least 500 ml | refrigerate |
| Ascitic fluid | at least 500 ml | refrigerate |
| Lymph node | minced in RPMI | refrigerate |
| Fine needle aspirate | suspend in RPMI | refrigerate |
For CSF and other specimens not listed above, call the laboratory for handling instructions.
Please notify the laboratory before sending a specimen.
